PK1109

PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb

liquid, Calbiochem^®

• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: liquid
• non contiene: preservative
• Reattività contro le specie: mouse, rat, chicken, human, Xenopus
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pTyr416)
• Informazioni sul gene: human ... SRC(6714)

Descrizione

Descrizione generale

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁶.

Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁶ in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.

This PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb is validated for use in Immunoblotting for the detection of Src Family (pTyr⁴¹⁶).

Immunogeno

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁴¹⁶ phosphorylation site of human Src

Applicazioni

Immunoblotting (1:1000)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Ricostituzione

Do not aliquot.

Risultati analitici

Positive Control
COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma

Altre note

May also recognize other Src family kinases phosphorylated at equivalent sites. The immunogen sequence is 100% conserved in chicken, rat, and Xenopus, but cross-reactivity has not been tested. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Thomas, S.M. and Brugge, J.S. 1997. Annu. Rev. Cell Dev. Biol.13, 513.
Chow, L.M. et al. 1993. Nature365, 156.
Molina, T.J., et al. 1992. Nature357, 161.
Straus, D.B. and Weiss, A. 1992. Cell70, 585.
Yamanashi, Y., et al. 1991. Science251, 192.
Yamanashi, Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 6538.
Hunter, T. 1987. Cell49, 1.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2