MABN1571

Anti-ARHGEF9 Antibody, clone 4D7.1

clone 4D7.1, from mouse

• Sinonimo/i: Rho guanine nucleotide exchange factor 9, Collybistin, PEM-2 homolog, Rac/Cdc42 guanine nucleotide exchange factor 9
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 4D7.1, monoclonal
• Reattività contro le specie: mouse
• Reattività contro le specie (prevista in base all’omologia): human (based on 100% sequence homology)
• tecniche: western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_056000
• N° accesso UniProt: O43307
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ARHGEF9(23229)

Descrizione

Descrizione generale

Rho guanine nucleotide exchange factor 9 (UniProt O43307; also known as Collybistin, PEM-2 homolog, Rac/Cdc42 guanine nucleotide exchange factor 9) is encoded by the ARHGEF9 (also known as ARHDH9, EIEE8, KIAA0424) gene (Gene ID 23229) in human. Guanine nucleotide exchange factors (GEFs) are cytoplasmic proteins that activate GTPases by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP). ARHGEF9 acts as a specific GEF for CDC42 and promotes formation of GPHN clusters. In the brain tissue it interacts with the receptor-anchoring protein gephyrin to activate dc42, which is known to regulate actin cytoskeleton dynamics. Its binding to gephyrin can also modulate GABAergic and glycinergic neurotransmission at the synapse. Abnormalities in its expression have been linked to epileptic encephalopathy, early infantile, 8.

Specificità

Clone 4D7.1 targets ARHGEF9 C-terminal region present in human isoforms 1/2/3 and mouse isoforms 1/2/4 reported by UniProt (O43307 and Q3UTH8). Reactivity toward mouse isoform 3 is not expected.

Immunogeno

GST-tagged human ARHGEF9 C-terminal fragment.

Applicazioni

Anti-ARHGEF9 Antibody, clone 4D7.1, Cat. No. MABN1571, is a highly specific mouse monoclonal antibody that targets ARHGEF9 and has been tested in Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:500 dilution from a representative lot detected ARHGEF9 in 10 µg of mouse whole brain tissue lysate.

Qualità

Evaluated by Western Blotting in mouse hypothalamus tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ARHGEF9 in 10 µg of mouse hypothalamus tissue lysate.

Descrizione del bersaglio

~61 kDa observed. 60.98/54.97/49.46 kDa (human isoform 1/2/3) and 60.93/58.62/60.86 kDa (mouse isoform 1/2/4) calculated. Human isoforms 1 and 2 are also known as CB3(SH3+) and CB3(SH3-). Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified.

Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable