MABF2674

Anti-IL-18 Antibody

purified antibody, clone 11–4.1, from mouse

• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 11–4.1, monoclonal
• PM: calculated mol wt 22.33 kDa; observed mol wt ~19 kDa
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 100 μL
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: -10 to -25°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Specificità

Clone 11-4.1 is a mouse monoclonal antibody that detects human IL-18 protein. It targets an epitope within 6 amino acids from the N-terminal half.

Immunogeno

His-tagged full-length active recombinant human Interleukin-18 (without the propeptide).

Applicazioni

Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected IL-18 in HeLa cell lysate.Tested ApplicationsImmunoprecipitation Analysis: A representative lot immunoprecipitated IL-18 in Immunoprecipitation applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Immunocytochemistry Analysis: A representative lot detected IL-18 in Immunocytochemistry applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Western Blotting Analysis: A representative lot detected IL-18 in Western Blotting applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected IL-18 in HeLa cells.Inhibition Assay: A representative lot inhibited IL-18 involved interferon release from KG-1 cells.(Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-18, clone 11 4.1, Cat. No. MABF2674, is a mouse monoclonal antibody that detects Interleukin-18 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.

Descrizione del bersaglio

Interleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor a (IL-18a) to form a low affinity binary complex that recruits IL-18b to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon  synthesis from T-helper 1 and natural killer cells. Clone 11-4.1 is shown to block IL-18 evolved interferon release in a dose-dependent manner (IC50 = 14.2 nM). (Ref.: Nariai, Y., et al. (2019). Arch. Biochem. Biophys. 663; 71-82).

Stato fisico

Purified mouse monoclonal antibody IgG2b in PBS without preservatives.

Stoccaggio e stabilità

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable