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MABF2248

Sigma-Aldrich

Anti-EBV LMP1 Antibody, clone S12

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Forma dell’anticorpo

purified antibody

Clone

S12
monoclonal

Purificato mediante

using protein G

Reattività contro le specie

mouse, virus

Concentrazione

(Please refer to lot specific datasheet.)

tecniche

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2aκ

N° accesso UniProt

Descrizione generale

A fusion protein (pKH548) consisting of 189 amino acids from the C-terminus region of LMP1 coupled to beta-galactosidase.

Specificità

Clone S12 is a mouse monoclonal antibody that specifically detects Latent membrane protein 1 (LMP1) in Epstein-Barr virus transformed cells.

Applicazioni

Immunocytochemistry Analysis: A representative lot detected EBV LMP1 in Immunocytochemistry applications (Xu, J., et. al. (2002). J Virol. 76(8):4080-6).

Flow Cytometry Analysis: A representative lot detected EBV LMP1 in Flow Cytometry applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Western Blotting Analysis: A representative lot detected EBV LMP1 in Western Blotting applications (Choi, I.K., et. al. (2018). Proc Natl Acad Sci USA. 115(4):E686-E695; Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65; Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Immunofluorescence Analysis: A representative lot detected EBV LMP1 in Immunofluorescence applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Immunohistochemistry Analysis: A representative lot detected EBV LMP1 in Immunohistochemistry applications (Brink, A.A., et. al. (1997). J Clin Pathol. 50(11):911-8; Khabir, A., et. al. (2005). Virol J. 2:39).

Immunoprecipitation Analysis: A representative lot immunoprecipitated EBV LMP1 in Immunoprecipitation applications (Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65).

Qualità

Evaluated by Western Blotting in Raji cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected LMP1 in lysates from Raji cells.

Proprietà fisiche

~59 kDa observed; 41.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Virus transformed human cells.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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