MABE1985

Anti-Gemin5 Antibody, clone 1E12

• Sinonimo/i: Gem-associated protein 5
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 1E12, monoclonal
• PM: calculated mol wt 168.59 kDa; observed mol wt ~170 kDa
• Purificato mediante: using protein G
• Reattività contro le specie: human, human, monkey
• Reattività contro le specie (prevista in base all’omologia): porcine
• Confezionamento: antibody small pack of 100 μg
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1
• Sequenza dell’epitopo: C-terminal
• N° accesso ID proteina: NP_001239085
• N° accesso UniProt: Q8TEQ6
• Condizioni di spedizione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GEMIN5(25929)

Descrizione

Descrizione generale

Gem-associated protein 5 (UniProt: Q8TEQ6; also known as Gemin5) is encoded by the GEMIN5 gene (Gene ID: 25929) in human. Survival of motor neurons (SMN) protein is known to form a stable complex with a group of proteins named gemins. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs) and thereby plays an important role in the splicing of cellular pre-mRNAs. Gemin5, a highly conserved, multi-functional protein, is a component of the core SMN complex and is found in the nucleoplasm and nuclear bodies known as Gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. It is also abundantly present in the cytoplasm. It plays a role in capturing snRNA into SMN complexes in the cytoplasm for transport into the nucleus. However, in the nucleus it is easily detached from the SMN-Gemin complex. Gemin5 is reported to physically bind to snRNA via a specific AU5-6 sequence located within the highly conserved Sm site and is flanked by a short stem loop that assists in delivery to the SMN complex. The specific snRNP code helps Gemin5 in distinguishing these snRNAs from other forms of cellular RNAs. Gemin5 contains 13 WD repeat domains that mediate its binding to U1 and U4 snRNA. WD domains are also reported to mediate binding to the 7-methylguanosine cap that is found on mRNA and snRNA molecules. Mutations in GEMIN5 gene are known to cause a distinct neurological cerebellar ataxia syndrome, through altered snRNP complex assembly. (Ref.: Kour, S., et al. (2021). Nat. Commun. 12; Article 2558; Hao, L., et al. (2007). BMC Cell Biol. 8:28).

Specificità

Clone 1E12 is a mouse monoclonal antibody that detects Gem-associated protein 5 (Gemin5). It targets an epitope within the C-terminal region.

Immunogeno

His-tagged recombinant fragment corresponding to 292 amino acids form the C-terminal region of human Gem-associated protein 5 (Gemin5).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in COS-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in HeLa cell lysate.

Immunocytochemistry Analysis: A representative lot detected Gemin5 in Immunocytochemistry application (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28; Schilling, M., et al. (2021). Cell Rep. 35(12); 109277).

Western Blotting Analysis: A representative lot detected Gemin5 in Western Blotting applications (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Gemin5, clone 1E12 , Cat. No. MABE1985, is a mouse monoclonal antibody that detects Gemin5 and is tested for use in Immunocytochemistry and Western Blotting.

Stato fisico

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Recommended storage: +2°C to +8°C.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable