MABE1012

Anti-E2F-7 Antibody, clone 14C3.2

clone 14C3.2, from mouse

• Sinonimo/i: Transcription factor E2F7, E2F-7
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• Clone: 14C3.2, monoclonal
• application: ICC; WB
• Reattività contro le specie: mouse, human
• tecniche: immunocytochemistry: suitable; western blot: suitable

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 14C3.2, monoclonal
• Reattività contro le specie: mouse, human
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_976328
• N° accesso UniProt: Q96AV8
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... E2F7(144455)

Descrizione

Descrizione generale

Transcription factor E2F7 (UniProt Q96AV8; also known as E2F-7) is encoded by the E2F7 gene (Gene ID 144455) in human. The E2F family of transcription factors (E2F) regulate genes involved in cell proliferation, differentiation and apoptosis. E2F1, 2, 3a function as transcription activators, while other family members (E2F3b, 4, 5, 6, 7, 8) are known as transcription repressors. Upregulated E2F7 is found in tamoxifen-resistant breast cancer cells, where it is responsible for transcriptional repression of miR-15a/16. Exogenous expression of miR-15a/16 or silencing E2F7 re-sensitizes breast cancer cells to tamoxifen-induced cell cycle arrest and apoptosis. Upregulated E2F7 expression is also reported in cutaneous squamous cell carcinomas (CSCC) and and head and neck SCC (HNSCC), where E2F7-dependent Sphk1 overexpression in SCC results in an elevated production of the antiapoptotic phospholipid, sphingosine-1-phosphate (S1P), which in turn invokes anthracycline resistance via activation of the PI3K/AKT pathway.

Specificità

Clone 14C3.2 recognizes an internal epitope of human E2F-7 present in all three spliced isoforms reported by UniProt (Q96AV8).

Immunogeno

Epitope: Internal (C-terminal half).

GST-tagged recombinant human E2F-7 internal fragment.

Applicazioni

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected E2F-7 in A431, HeLa, HepG2 and NIH/3T3 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

This Anti-E2F-7 Antibody, clone 14C3.2 is validated for use in Western Blotting, Immunocytochemistry for the detection of E2F-7.

Qualità

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected E2F-7 in 10 µg of HEK293 cell lysate.

Descrizione del bersaglio

~100 kDa observed. 99.89 kDa (isoform 1; E2F7b), 80.87 kDa (isoform 2; E2F7a), 98.35 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable