MABC91
Anti-Atg18 (WIPI-2) Antibody, clone 2A2
clone 2A2, from mouse
Sinonimo/i:
WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
2A2, monoclonal
Reattività contro le specie
human
tecniche
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG1κ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... WIPI2(26100)
Descrizione generale
Atg18, also known as WIPI-2, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.
Specificità
This antibody recognizes Atg18 at the C-terminus.
Immunogeno
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of human Atg18.
Applicazioni
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Atg18 in human colorectal adenocarcinoma tissue.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Atg18 (WIPI-2) Antibody, clone 2A2 is validated for use in WB, IH(P) for the detection of Atg18 (WIPI-2).
Qualità
Evaluated by Western Blot in human testis tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate.
Descrizione del bersaglio
~49 kDa observed.
6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa
6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Risultati analitici
Control
Human testis tissue lysate
Human testis tissue lysate
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Shusaku Shibutani et al.
The Journal of biological chemistry, 292(44), 18052-18061 (2017-08-16)
The mechanistic target of rapamycin complex 1 (mTORC1) is a master regulator of protein synthesis and potential target for modifying cellular metabolism in various conditions, including cancer and aging. mTORC1 activity is tightly regulated by the availability of extracellular amino
Hannah C Dooley et al.
Molecular cell, 55(2), 238-252 (2014-06-24)
Mammalian cell homeostasis during starvation depends on initiation of autophagy by endoplasmic reticulum-localized phosphatidylinositol 3-phosphate (PtdIns(3)P) synthesis. Formation of double-membrane autophagosomes that engulf cytosolic components requires the LC3-conjugating Atg12-5-16L1 complex. The molecular mechanisms of Atg12-5-16L1 recruitment and significance of PtdIns(3)P
Yasuhiro Yamamoto et al.
Nature communications, 12(1), 3292-3292 (2021-06-04)
Autophagy regulates primary cilia formation, but the underlying mechanism is not fully understood. In this study, we identify NIMA-related kinase 9 (NEK9) as a GABARAPs-interacting protein and find that NEK9 and its LC3-interacting region (LIR) are required for primary cilia
Ji-Man Park et al.
Autophagy, 12(3), 547-564 (2016-04-06)
ULK1 (unc-51 like autophagy activating kinase 1), the key mediator of MTORC1 signaling to autophagy, regulates early stages of autophagosome formation in response to starvation or MTORC1 inhibition. How ULK1 regulates the autophagy induction process remains elusive. Here, we identify
Norito Tamura et al.
FEBS letters, 591(23), 3819-3830 (2017-11-08)
ATG2 is one of the autophagy-related (ATG) proteins essential for autophagosome formation and localizes to isolation membranes and lipid droplets in mammalian cells. Here, we investigated the requirement of regions in ATG2A for its organellar localization and function. The N-terminal
Articoli
Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
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