MABC1216

Anti-ARHGAP36 Antibody, clone 12D10.1

clone 12D10.1, from mouse

• Sinonimo/i: Rho GTPase-activating protein 36
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 12D10.1, monoclonal
• Reattività contro le specie: human
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_659404
• N° accesso UniProt: Q6ZRI8
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ARHGAP36(158763)

Descrizione

Descrizione generale

Rho GTPase-activating protein 36 (UniProt Q6ZRI8) is encoded by the ARHGAP36 gene (Gene ID 158763) in human. ARHGAP36 is a Rho GAP family member that acts as a potent hedgehog (Hh) pathway positive regulator. ARHGAP36 is reported to functionally associate with Hh pathway negative regulator Sufu (suppressor of fused) and ARHGAP36 overexpression results in reduced Gli3R levels, Gli2 ciliary accumulation, and Gli1 expression, in a Smo-independent manner. ARHGAP36 is produced with a signal peptide sequence (a.a. 1-40), which is removed posttranslationally to yield the mature protein with a Rho-GAP domain (a.a. 226-426). Unlike other Rho GAP domain-containing proteins, ARHGAP36 lacks the arginine finger motif that stimulates GTPase hydrolysis and GAP domain mutants can retain Gli-activating activity. ARHGAP36 isoforms are up-regulated in a subset of medulloblastomas, where they mediate noncanonical Gli activation by inhibiting Sufu.

Specificità

Clone 12D10.1 epitope lies within a C-terminal region present in all five human ARHGAP36 spliced isoforms reported by UniProt (Q6ZRI8).

Immunogeno

GST-tagged recombinant human ARHGAP36 C-terminal fragment.

Applicazioni

Detect Arhgap36 using this mouse monoclonal Anti-ARHGAP36, clone 12D10.1 Antibody, Cat. No. MABC1216, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ARHGAP36 in human prostate cancer, adrenal gland and pancreas tissue sections.

Research Category
Apoptosis & Cancer

Qualità

Evaluated by Western Blotting in Arhgap36 recombinant protein.

Western Blotting Analysis: A 1:500 dilution of this antibody detected 50 ng of GST-tagged recombinant human ARHGAP36 C-terminal fragment.

Descrizione del bersaglio

57.57/61.66 kDa (isoform 1 mature/proform), 57.57/58.34 kDa (isoform 2 mature/proform), 46.23 kDa (isoform 3), 57.57/60.57 kDa (isoform 4 mature/proform), 57.57 kDa (isoform 5).

Stato fisico

Format: Purified

Protein G purified.

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable