MAB3786-C

Anti-APC Antibody, CT, clone C-APC Antibody 28.9, Ascites Free

clone C-APC 28.9, from mouse

• Sinonimo/i: Adenomatous polyposis coli protein, Deleted in polyposis 2.5, Protein APC
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: C-APC 28.9, monoclonal
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_000029
• N° accesso UniProt: P25054
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... APC(324)

Descrizione

Descrizione generale

Adenomatous polyposis coli protein (UniProt P25054; also known as Deleted in polyposis 2.5, Protein APC) is encoded by the APC (also known as DP2.5) gene (Gene ID 324) in human. The multifunctional tumor suppressor APC is a large scaffold protein that contains multiple armadillo repeat (ARM) domains (a.a. 453-767 of human APC spliced isoform 1) for mediating various cellular functions, including Wnt signal regulation, cell adhesion, and chromosomal segregation during mitosis. APC, AXIN, casein kinase 1 (CK1) and glycogen synthase kinase 3 (GSK3) constitute the core proteins of the β-catenin destruction complex that functions as the negative regulator against Wnt signaling transduction. Mice with Apc gene mutations develop adenomas in the intestine due to Wnt hyperactivation, and functional loss of APC due to gene mutations is the hallmark of human colorectal cancers (CRC). APC also directs cholinergic synapse assembly between neurons and is commonly employed as a marker for monitoring stem cells neuronal differentiation.

Specificità

Recognizes human APC, molecular weight is approximately 310 kDa.

Immunogeno

Epitope: C-terminal region.

Human APC C-terminal fusion with MBP (maltose binding protein).

Applicazioni

Anti-APC Antibody, CT, clone C-APC Antibody 28.9, Ascites Free is an antibody against APC for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence, Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC in human kidney and cardiac muscle.
Immunocytochemistry Analysis: A representative detected an increased APC expression in human eyelid adipose-derived stem cells (hEASCs) after neuroinduction at passage 8 (Zhou, J., et al. (2014). J. Cell. Mol. Med. 18(2):326-343).
Immunofluorescence Analysis: A representative lot detected a granular APC staining pattern at a subapical region of epithelial cells in fallopian mucosa by fluorescent immunohistochemistry staining of paraffin-embedded, paraformaldehyd-fixed fallopian tube tissue sections (Kessler, M., et al. (2012). Am. J. Pathol. 180(1):186-198).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualità

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC in human cerebral cortex tissue.

Descrizione del bersaglio

311.6 kDa calculated.

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable