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Documenti fondamentali

MAB3554

Sigma-Aldrich

Anti-Cytokeratin 7 Antibody, clone OV-TL 12/30

clone OV-TL 12/30, Chemicon®, from mouse

Sinonimo/i:

Anti-Anti-CD20, Anti-Anti-CK-20, Anti-Anti-CK20, Anti-Anti-K20, Anti-Anti-KRT21

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

OV-TL 12/30, monoclonal

Reattività contro le specie

human

Produttore/marchio commerciale

Chemicon®

tecniche

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotipo

IgG1

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... KRT7(3855)

Descrizione generale

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry.

Specificità

Cytokeratin 7. Reacts with specific glandular-type epithelia, and most carcinomas derived there from.

Immunogeno

Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.

Applicazioni

Anti-Cytokeratin 7 Antibody, clone OV-TL 12/30 detects level of Cytokeratin 7 & has been published & validated for use in FC, WB, IC, IH(P).
Immunoblotting

Immunohistochemistry on frozen tissue sections fixed with methanol. The antibody is also effective on paraffin embedded tissue sections that have been pretreated with 0.1% pronase/0.1% trypsin in PBS, 0.5% pepsin in 0.01N HCl or microwave pretreatment.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytokeratins

Linkage

Replaces: MABT150

Stato fisico

Format: Purified
Liquid in PBS containing 0.09% sodium azide.

Stoccaggio e stabilità

Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Ze Wu et al.
Cell communication and signaling : CCS, 20(1), 89-89 (2022-06-16)
Growth differentiation factor-11 (GDF-11), also known as bone morphogenetic protein-11, belongs to the transforming growth factor-beta superfamily. GDF-11 was first identified as an important regulator during embryonic development. Increasing evidence has demonstrated that GDF-11 regulates the development of various organs
Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas.
van Niekerk, C C, et al.
The American Journal of Pathology, 138, 455-463 (1991)
Yuyang Tang et al.
Cell reports, 30(13), 4528-4539 (2020-04-03)
Radical cure of HIV-1 (HIV) is hampered by the establishment of HIV reservoirs and persistent infection in deep tissues despite suppressive antiretroviral therapy (ART). Here, we show that among HIV-positive women receiving suppressive ART, cells from placental tissues including trophoblasts
Hong-Jin Zhao et al.
Cellular signalling, 67, 109489-109489 (2019-12-02)
Both bone morphogenetic protein 2 (BMP2) and WNT/β-catenin signaling promote human trophoblast cell invasion. BMP2 has been shown to up-regulate bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) in granulosa cells. Besides, studies indicate BAMBI is a positive regulator for
Jiali You et al.
Frontiers in cell and developmental biology, 9, 607332-607332 (2021-02-23)
Embryo implantation and trophoblast invasion are principal limiting factors of pregnancy establishment. Aberrant embryo development or improper trophoblast differentiation and invasion may lead to various unfavorable pregnancy-related outcomes, including early pregnancy loss (EPL). Our clinical data show that the serum

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