MAB2600

Anti-Glypican-1 Antibody, clone 4D1

clone 4D1, from mouse

• Sinonimo/i: glypican 1, glypican proteoglycan 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 4D1, monoclonal
• Reattività contro le specie: human, rat
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_002072
• N° accesso UniProt: P35052
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GPC1(2817) ; rat ... Gpc1(58920)

Descrizione

Descrizione generale

Heparan sulfate proteoglycans (HSPGs) can be divided into general categories of
cell-associated forms, including syndecans, glypicans, and secreted extracellular matrix forms, such as perlecan. Glypicans contain six family members to date and are proteins bound to the outer surface of the plasma membrane by a glycosyl-phosphatidylinositol anchor and involved in cellular adhesion and migration and function as accessory molecules for signaling receptors, (Filmus, 2008 & Gui, 2006). Current publications indicate the main function of membrane-attached glypicans is to regulate the signaling of Wnts, Hedgehogs, fibroblast growth factors and bone morphogenetic proteins (BMPs), (Filmus, 2008).
Glypican-1 (GPC1) is over-expressed in human gliomas and appears to have a greater capability of promoting FGF-2 signaling in some gliomas versus HSPGs in normal astrocytes. Additional observations suggest that GPC1 can impact the growth of pancreatic cancer cells by attenuating tumor angiogenesis, (Whipple, 2008).

Specificità

MAB2600 recognizes glypican-1.

Immunogeno

Epitope: Full Length

Full length recombinant protein.

Applicazioni

Anti-Glypican-1 Antibody, clone 4D1 detects level of Glypican-1 & has been published & validated for use in IH(P) & WB.

Immunohistochemistry:
A 1:50 dilution of a previous lot detected strong cytoplasmic staining of tumor cells in formalin-fixed, paraffin-embedded gangliobastoma tissue sample. There was negative staining of normal, uninvolved cells.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Qualità

Routinely evaluated by Western blot on human PC-12 cell lysate using 0.5 µg/mL of Anti-Glypican-1 at 1 mg/mL.

Descrizione del bersaglio

Recognizes Glypican-1 at ~ 59 kDa

Stato fisico

Format: Purified

Protein G Purified

Purified in 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% NaN3.

Stoccaggio e stabilità

Stable for 1 year at 2-8ºC from date of receipt.

Risultati analitici

Control
PC-12 cell lysate.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable