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MAB1522

Sigma-Aldrich

Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7

ascites fluid, clone CGA7, Chemicon®

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

ascites fluid

Clone

CGA7, monoclonal

Reattività contro le specie

human, rat, chicken, rabbit, monkey

Produttore/marchio commerciale

Chemicon®

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotipo

IgG2a

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Specificità

Antibody recognizes actin isotypes alpha and gamma of smooth muscle cells, but not skeletal or cardiac muscle cells. Reactive with myoepithelium, but nonreactive with other mesenchymal and all epithelial cells. The antibody labels eiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinomas or lymphoma.

Immunogeno

Chicken gizzard actin
Epitope: smooth muscle gamma & alpha actin

Applicazioni

Detect Actin using this Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7 validated for use in WB, IH, IH(P).
Western Blot: 1:1000 (approximately 43 kDa)

Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation)

Optimal dilutions must be determined by the end user.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Kristine H Kurtz et al.
PloS one, 9(4), e94082-e94082 (2014-04-09)
The mechanisms that control phasic and tonic contractions of lymphatic vessels are poorly understood. We hypothesized that rho kinase ROCK, previously shown to increase calcium (Ca2+) sensitivity in vascular smooth muscle, enhances lymphatic contractile activity in a similar fashion. Contractions
Smooth muscle actin expression during rat gut development and induction in fetal skin fibroblastic cells associated with intestinal embryonic epithelium.
Kedinger, M, et al.
Differentiation, 43, 87-97 (1990)
Xin Yao et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 13(1), 161-169 (2007-01-04)
Intratumoral microvascular density (MVD) has been controversial as an indicator of prognosis in clear cell renal cell carcinoma (CCRCC). Classification of the intratumoral blood vessels based on differential expressions of blood vessel markers has not been correlated with patient prognosis
Cultured circular smooth muscle from the rabbit colon.
Kao, H W, et al.
In Vitro Cellular & Developmental Biology, 24, 787-794 (1988)
Endogenous gamma-aminobutyric acid modulates tonic guinea pig airway tone and propofol-induced airway smooth muscle relaxation.
G Gallos, NR Gleason, L Virag, Y Zhang, K Mizuta, RA Whittington, CW Emala
Anesthesiology null

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