ABN98

Anti-Neurexin-1-alpha Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Neurexin-1-alpha, Neurexin I-alpha, Neurexin1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: mouse, human, rat
• Reattività contro le specie (prevista in base all’omologia): chimpanzee (based on 100% sequence homology), canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology)
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NP_004792
• N° accesso UniProt: Q9ULB1
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NRXN1(9378)

Descrizione

Descrizione generale

Neurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion (see review by Missler & Sudhof, 1998). Antibody Anti-Neurexin-1-alpha (ABN35) was made against a peptide immunogen corresponding to a stretch of amino acids in the extracellular domain of human Neurexin-1-alpha (Uniprot Q9ULB1). Although the theoretical full length of Neurexin-1-alpha is 161 kDa, the protein detected in human brain lysates may show a band around 160 kDa and additional bands around 45 kDa as a possible result of the posttranslational processing previously reported.

Specificità

This antibody recognizes Neurexin-1-alpha at the extracellular laminin G-like 3 domain.

Immunogeno

Epitope: Extracellular laminin G-like 3 domain

KLH-conjugated Linear peptide corresponding to human Neurexin-1-alpha at the extracellular laminin G-like 3 domain.

Applicazioni

Detect Neurexin-1-alpha using this Anti-Neurexin-1-alpha Antibody validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Neurexin-1-alpha in human brain tissue.

Research Category
Neuroscience

Research Sub Category
Adhesion (CAMs)

Qualità

Evaluated by Western Blot in human cerebellum tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Neurexin-1-alpha on 10 µg of human cerebellum tissue lysate.

Descrizione del bersaglio

~ 45 kDa Observed At higher concentrations or longer exposures, in certain lysates, a band at ~150kD can be seen that corresponds to the expected full length Mw.
UniProtKB/Swiss-Prot Q9ULB1 (NRX1A_HUMAN) entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. Reference (Accolade/Related Documents) TIG JANUARY 1998 VOL. 14 No. 1 indicates that multiple neurexin isoforms are generated from three genes by alternative promoters and alternative splicing.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Human cerebellum tissue lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable