ABN1475

Anti-Serotonin receptor 3A/HTR3A

from rabbit, purified by affinity chromatography

• Sinonimo/i: 5-hydroxytryptamine receptor 3A, 5-HT-3, 5-HT3-A, 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NP_000860
• N° accesso UniProt: P46098
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... HTR3A(3359)

Descrizione

Descrizione generale

5-hydroxytryptamine receptor 3A (UniProt P46098; also known as 5-HT-3, 5-HT3-A, 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor) is encoded by the HTR3A (also known as 5HT3R, HTR3) gene (Gene ID 3359) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-hydroxytryptamine receptor 3 (5-HT3R) is a ligand-gated ion channel involved in many neurophysiological functions and is widely expressed throughout the brain and in peripheral organs. Five different subunits (A through E) have been identified, with the homopentameric 5-HT3A being the most studied form. 5-HT3A is produced with a signal peptide sequence (a.a. 1-23), which is removed posttranslationally to yield the mature 4-transmembrane (a.a. 242-268, 274-292, 303-321, 456-475) ion channel with both its N- and C-terminal ends exposed extracellularly (a.a. 24-241 and 476-478).

Specificità

This rabbit polyclonal antibody detects human Serotonin receptor 3A/HTR3A by targeting a sequence within the second cytoplasmic domain.

Immunogeno

KLH-conjugated linear peptide corresponding to a sequence from the second cytomplasmic domain of human Serotonin receptor 3A/HTR3A.

Applicazioni

Anti-Serotonin receptor 3A/HTR3A Antibody, Cat. No. ABN1475, is a highly specific rabbit polyclonal antibody that targets HTR3A and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected serotonin receptor 3A/HTR3A in human prostate cancer, as well as in normal cerebral cortex, heart and tonsil tissue sections.

Research Category
Neuroscience

Qualità

Evaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected serotonin receptor 3A/HTR3A in 10 µg of human cerebral cortex tissue lysate.

Descrizione del bersaglio

~55 kDa observed. 52,79/55.28 kDa (mature/pro-isoform 1; 5-HT3R-AS), 55.90/58.39 kDa (mature/pro-isoform 2; 5-HT3R-AL), 52.79/53.76 kDa (mature/pro-isoform 3), 52.79/55.89 kDa (mature/pro-isoform 4), 55.90/59.00 kDa (mature/pro-isoform 5). Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable