ABE394

Anti-Cohesin subunit SA-2 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): equine (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_006594
• N° accesso UniProt: Q8N3U4
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... STAG2(10735)

Descrizione

Descrizione generale

The cohesin subunit SA-2 is a part of the cohesin complex. This complex is comprised of a heterodimer between SMC1 and SMC3 proteins, as well as RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a large ring that can trap sister chromatids after DNA replication. During prophase, the cohesion complexes dissociate from the chromatin due to the phosphorylation by PLK1 (Polo-like kinase) with the exception at the centromeres where the cohesion complexes remain. Then during anaphase, the RAD21 subunit is cleaved which causes the dissociation of the complex from chromosomes, allowing chromosome separation.

Specificità

Other homologies: Mouse and Rat (93%-14/15aa).

Immunogeno

KLH-conjugated linear peptide corresponding to human Cohesin Subunit SA-2.

Applicazioni

Detect Cohesin subunit SA-2 using this Anti-Cohesin subunit SA-2 Antibody validated for use in 0.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Cohesin Subunit SA-2 in A431 and HeLa cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cohesin Subunit SA-2 from HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Qualità

Evaluated by Western Blot in HeLa cell nuclear extract.

Western Blot Analysis: 0.2 µg/mL of this antibody detected Cohesin Subunit SA-2 in 10 µg of HeLa cell nuclear extract.

Descrizione del bersaglio

~140 kDa observed. An uncharacterized band may appear at ~35 kDa in some lysates. Uncharacterized bands may appear at ~40 kDa and ~30 kDa in Immunoprecipitation.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa cell nuclear extract

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable