ABE1958
Anti-Zic1/2
from rabbit
Sinonimo/i:
Zinc finger protein of the cerebellum 1
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
unpurified
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
human, mouse
tecniche
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
ambient
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
mouse ... Zic1(22771)
Descrizione generale
Zinc finger protein ZIC 1 (UniProt: P46684; also known as Zinc finger protein of the cerebellum 1) is encoded by the Zic 1 (also known as Zic) gene (Gene ID: 22771) in murine species. Zic 1 is a member of the GLI C2H2-type zinc-finger protein family. Zic 1 is expressed in the central nervous system and in osteoblasts. A high level expression is seen in the cerebellum, while a low level expression is seen in the olfactory bulb, diencephalon, and brainstem. It acts as a transcriptional activator that is involved in neurogenesis and plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. The C2H2-type 3, 4 and 5 zinc finger domains of Zic 1 are considered to be essential for transcription activation and for Zic 1 interaction with MyoD family inhibitor domain-containing (MDFIC) protein. Zic 1 is also shown to be involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). It plays a role in the regulation of MF axon pathway choice and promotes MF migration towards ipsilaterally-located cerebellar territories.
Specificità
This polyclonal antibody detects Zic1 protein in mouse and human.
Immunogeno
A linear peptide corresponding to 20 amino acids from the C-terminal end of mouse Zic 1 protein.
Epitope: C-terminus
Applicazioni
Anti-Zic1/2, Cat. No. ABE1958, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein ZIC 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, and Western Blotting.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Zic1/2 in mouse cerebellum (Frank, C.L., et. al. (2015). Nat Neurosci. 18(5):647-56).
Immunofluorescence Analysis: A representative lot detected Zic1/2 in postmitotic granule cells (Kim, J.Y., et. al. (2003). Dev Biol. 263(1):50-66) and adult mouse cerebellum (Koirala, S., et. al. (2009). J Neurosci. 29(23):7439-49).
Immunocytochemistry Analysis: A representative lot detected Zic1/2 in Immunocytochemistry applications (Kenney, A.M., et. al. (2000). Mol Cell Biol. 20(23):9055-67).
Immunofluorescence Analysis: A representative lot detected Zic1/2 in postmitotic granule cells (Kim, J.Y., et. al. (2003). Dev Biol. 263(1):50-66) and adult mouse cerebellum (Koirala, S., et. al. (2009). J Neurosci. 29(23):7439-49).
Immunocytochemistry Analysis: A representative lot detected Zic1/2 in Immunocytochemistry applications (Kenney, A.M., et. al. (2000). Mol Cell Biol. 20(23):9055-67).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Qualità
Evaluated by Western Blotting in human cerebellum tissue lysate.
Western Blotting Analysis: 1:500 dilution of this antibody detected Zic1/2 in 10 µg of human cerebellum tissue lysate.
Western Blotting Analysis: 1:500 dilution of this antibody detected Zic1/2 in 10 µg of human cerebellum tissue lysate.
Descrizione del bersaglio
~53 kDa observed; 48.33 kDa calculated for Zic1; 54.95 kDa calculated for Zic2. Uncharacterized bands may be observed in some lysate(s).
Stato fisico
Format: Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Stoccaggio e stabilità
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Altre note
Concentration: Please refer to lot specific datasheet.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Non trovi il prodotto giusto?
Prova il nostro Motore di ricerca dei prodotti.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.