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Key Documents

AB5467

Sigma-Aldrich

Anti-AANAT Antibody

serum, Chemicon®

Sinonimo/i:

Arylalkylamine N-acetyltransferase, SNAT, Serotonin Acetylase, EC 2.3.1.87

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

serum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

rat, sheep, fish, human, bovine

Produttore/marchio commerciale

Chemicon®

tecniche

western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... AANAT(15)

Specificità

Recognizes AANAT (Arylalkylamine N-acetyltransferase). The antibody specifically recognizes the phosphorylated N-terminal PKA site of AANAT (pT31) (Ganguly et al. 2005). The antibody reacts with natural and recombinant protein.

Immunogeno

Synthetic peptide (phosphorylated) corresponding to amino acids near the N-terminous of rat AANAT conjugated to BSA. {Ganguly, S et al, 2005}.

Applicazioni

Anti-AANAT Antibody is an antibody against AANAT for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western Blot: 1:10,000-1:20,000 using ECL. Reacts with the ~23 kDa AANAT protein. Some reactivity may also be seen at ~97 kDa. Suggested blocking buffer is PBS with 10% non fat dry milk and 0.2% Tween-20. Suggested dilution buffer is PBS with 1 mg/mL BSA fraction V. Suggested incubation time is overnight at room temperature (~23°C).

Optimal working dilutions must be determined by the end user.

Stato fisico

Liquid rabbit serum containing no preservative.

Stoccaggio e stabilità

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Risultati analitici

Control
POSITIVE CONTROL:

Night adapted rat pineal gland. Expression is light sensitive; highest levels are obtained from animals at night.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Melatonin synthesis: 14-3-3-dependent activation and inhibition of arylalkylamine N-acetyltransferase mediated by phosphoserine-205.
Ganguly, Surajit, et al.
Proceedings of the National Academy of Sciences of the USA, 102, 1222-1227 (2005)
Andrzej Slominski et al.
European journal of biochemistry, 270(16), 3335-3344 (2003-08-06)
We showed expression of the tryptophan hydroxylase gene and of tryptophan hydroxylase protein immunoreactivity in mouse skin and skin cells. Extracts from skin and melanocyte samples acetylated serotonin to N-acetylserotonin and tryptamine to N-acetyltryptamine. A different enzyme from arylalkylamine N-acetyltransferase
Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
Erik Maronde,Anastasia Saade,Katrin Ackermann,Hany Goubran-Botros,Cecile Pagan,Roman Bux et al.
Journal of Pineal Research null
Jinglin Zhang et al.
Journal of pineal research, 65(1), e12481-e12481 (2018-02-27)
Melatonin is a key hormone that regulates circadian rhythms, metabolism, and reproduction. However, the mechanisms of melatonin synthesis and secretion have not been fully defined. The purpose of this study was to investigate the functions of the LIM homeobox transcription
Placental melatonin production and melatonin receptor expression are altered in preeclampsia: new insights into the role of this hormone in pregnancy.
Dave Lanoix,Pascale Guerin,Cathy Vaillancourt
Journal of Pineal Research null

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