482728
Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb
liquid, Calbiochem®
Sinonimo/i:
Anti-iNOS
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About This Item
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
liquid
contiene
≤0.1% sodium azide as preservative
Reattività contro le specie
mouse, rat, human
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
avoid repeated freeze/thaw cycles
Isotipo
IgG
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... NOS1(4842)
Descrizione generale
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa inducible nitric oxide synthase (iNOS) protein.
Recognizes the ~130 kDa iNOS protein.
This Anti-Nitric Oxide Synthase, Inducible Rabbit pAb is validated for use in Frozen Sections, WB, ICC, Paraffin Sections for the detection of Nitric Oxide Synthase, Inducible.
Immunogeno
Mouse
a synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
Applicazioni
Frozen Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500)
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
Undiluted serum.
Ricostituzione
Following initial thaw, aliquot and freeze (-20°C).
Risultati analitici
Positive Control
Raw 264.7 cells treated with IFNγ and LPS
Raw 264.7 cells treated with IFNγ and LPS
Altre note
Exhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.
Recommended Procedure for Immunocytochemistry of Anti-iNOS:
1. Grow cells in appropriate medium.
2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
3. Wash cells 3-5 min with phosphate buffered saline (PBS).
4. Permeabilize with 2% saponin for 10 min at room temperature.
5. Fix in 4% formalin for 10 min at room temperature.
6. Wash 3-5 min with PBS.
7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
8. Wash 3-5 min with PBS.
9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Recommended Procedure for Immunocytochemistry of Anti-iNOS:
1. Grow cells in appropriate medium.
2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
3. Wash cells 3-5 min with phosphate buffered saline (PBS).
4. Permeabilize with 2% saponin for 10 min at room temperature.
5. Fix in 4% formalin for 10 min at room temperature.
6. Wash 3-5 min with PBS.
7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
8. Wash 3-5 min with PBS.
9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Lowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA89, 6711.
Lyons, C., et al. 1992. J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991. Nature351, 714.
Lyons, C., et al. 1992. J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991. Nature351, 714.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
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