371818

G-Protein βγ Binding Peptide, mSIRK

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange.

• Sinonimo/i: G-Protein βγ Binding Peptide, mSIRK, mSIRK, myr-SIRKALNILGYPDYD-OH
• Formula empirica (notazione di Hill): C₉₃H₁₅₀N₂₀O₂₅
• Peso molecolare: 1948.31
• Codice UNSPSC: 12352200

Proprietà

• Livello qualitativo: 100
• Saggio: ≥95% (HPLC)
• Forma fisica: lyophilized
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Solubilità: DMSO: 5 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes α subunit dissociation without stimulating nucleotide exchange. Reported to cause a rapid activation of ERK1/2 (EC₅₀ ~2.5-5 µM) in RASM (rat arterial smooth muscle) and Rat2 cells. Promotes the phosphorylation of JNK and p38 MAP kinase. Also increases phospholipase C activity and causes Ca²⁺ release from internal stores.

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange. Shown to cause a rapid activation of ERK1/2 (EC₅₀ ~ 2.5 - 5 µM) in rat arterial smooth muscle and Rat2 cells. Also promotes the phosphorylation of JNK and p38 MAP kinase, phospholipase C and causes Ca²⁺ release from internal stores. Negative control is also available (Cat. No. 371820).

Azioni biochim/fisiol

Cell permeable: yes

EC50 ~ 2.5 - 5 µM in activation of ERK1/2 in rat arterial smooth muscle and Rat2 cells

Primary Target
N-myristoylated G-protein βγ selective binding

Product does not compete with ATP.

Reversible: no

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Sequenza

Myr-N-Ser-Ile-Arg-Lys-Ala-Leu-Asn-Ile-Leu-Gly-Tyr-Pro-Asp-Tyr-Asp-OH

Ricostituzione

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable