345749

GDF-8 Propeptide

≥90% (SDS-PAGE), lyophilized powder, mouse

• Codice UNSPSC: 12352203
• NACRES: NA.26

Proprietà

• product name: GDF-8 Propeptide, His•Tag^®, Mouse, Recombinant, GDF-8 Propeptide, His•Tag, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.
• Ricombinante: expressed in mouse NSO cells (myeloma cells)
• Livello qualitativo: 100
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: lyophilized
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Impurezze: ≤1.0 EU/μg protein Endotoxin
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Recombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.

Applicazioni

GDF-8 Propeptide, His•Tag, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

Azioni biochim/fisiol

ED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from 0.2 µm filtered PBS, BSA.

Ricostituzione

Following reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Altre note

Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144.
Thies, R.S. et al. 2001. Growth Factors18, 251.
Chang, H. et al. 2002. Endocrine Reviews23, 787.
McPherron, A.C. et al. 1997. Nature387, 83.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2