324715

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

• Sinonimo/i: Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
• Numero CAS: 72561-05-8
• Classificazione EC (Enzyme Commission): 3.4.21.50 (BRENDA, IUBMB)
• Numero MDL: MFCD02253020
• Codice UNSPSC: 12352202

Proprietà

• Grado: Proteomics Grade
• Livello qualitativo: 100
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: lyophilized
• Attività specifica: ≥200 units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Attenzione

Toxicity: Harmful (C)

Definizione di unità

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.

Stato fisico

Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Reconstitute in 50 µl of distilled H₂O.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany