203195

Protein A, Biotin Conjugate

This Protein A, Biotin Conjugate is validated for use in Solid Phase Immunoassay.

• Codice UNSPSC: 41116133
• NACRES: NA.41

Proprietà

• Livello qualitativo: 100
• Forma fisica: liquid
• contiene: ≤0.1% sodium azide as preservative
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Native protein from Straphylococcus Aureus, conjugated to biotin. Recognizes the Fc portions of immunoglobulins of various species.

Native protein A conjugated to biotin.

Applicazioni

Solid Phase Immunoassay (1:10,000)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 10 mM PBS, 1% BSA, pH 7.6.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Risultati analitici

A single band at MW 50,000 by SDS-PAGE

Altre note

The protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable