17-294
Rho Activation Assay Kit
PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.
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About This Item
Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Prodotti consigliati
Livello qualitativo
Produttore/marchio commerciale
Upstate®
tecniche
activity assay: suitable
affinity binding assay: suitable
N° accesso NCBI
Metodo di rivelazione
chemiluminescent
colorimetric
fluorometric
Condizioni di spedizione
dry ice
Informazioni sul gene
human ... RHOA(387)
Descrizione generale
A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.
Applicazioni
PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.
Confezionamento
Kit capacity: 30 assays
Componenti
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)
Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)
100X GDP, 100mM (Cat.# 20-177)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)
Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)
Qualità
Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Codice della classe di stoccaggio
10 - Combustible liquids
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