171263-M

AMPK Signaling Activator IX, F17

The AMPK Signaling Agonist, F17, also referenced under CAS 280112-24-5, controls the biological activity of AMPK. This small molecule/inhibitor is primarily used for Biochemicals applications.

• Sinonimo/i: AMPK Signaling Activator IX, F17, 4-hydroxy-1-isobutyl-N-(5-methylthiazol-2-yl)-2-oxo-1,2-dihydroquinoline-3-carboxamide
• Formula empirica (notazione di Hill): C₁₈H₁₉N₃O₃S
• Numero CAS: 280112-24-5
• Peso molecolare: 357.43
• Codice UNSPSC: 12352200

Proprietà

• Saggio: ≥95% (HPLC)
• Stato: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: off-white
• Solubilità: DMSO: 5 mg/mL, clear, colorless
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C18H19N3O3S/c1-10(2)9-21-13-7-5-4-6-12(13)15(22)14(17(21)24)16(23)20-18-19-8-11(3)25-18/h4-8,10,22H,9H2,1-3H3,(H,19,20,23); QSZAFLFWFQTCJK-UHFFFAOYSA-N

Descrizione

Descrizione generale

A cell-permeable 4-hydroxy-2-oxo quinoline carboxamide that acts as an agonist of AMPK signaling. It is shown to reduce fat storage in C. elegans (EC₅₀ ~ 0.1 µM) and reduce the expression level of fat-7 (a C. elegans ortholog of mammalian stearoyl-CoA desaturase-1), through aak-1 (an ortholog for the AMPK-α1 catalytic subunit) and K08F8.2 (a transcription factor) dependent mechanisms. In addition, it markedly reduces the number of lipid droplets in HepG2 human hepatocarcinoma cells at 25 µM, and elicits the phosphorylation and inactivation of acetyl coenzyme A carboxylase (ACC, the enzyme that catalyzes the rate-limiting step in de novo fatty acid synthesis). F17-induced ACC phosphorylation can be abrogated by simultaneous treatment with an AMPK inhibitor compound C (Cat. No. 171260).

Attenzione

Toxicity: Standard Handling (A)

Nota sulla preparazione

Warming to 55-60°C is required for complete solubilization.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Altre note

Lemieux, G.A., et al. 2011. Nat. Chem. Biol.7, 206.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable