17-10388

Di-Ubiquitin (Lys63-linked) Recombinant Protein

Native K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.

• Codice UNSPSC: 12352200
• eCl@ss: 32160405
• NACRES: NA.52

Proprietà

• Livello qualitativo: 100
• Saggio: >95% (reversed phase HPLC)
• PM: M_w ~17 kDa
• Produttore/marchio commerciale: Chemicon^®
• Condizioni di spedizione: dry ice

Descrizione

Descrizione generale

ANTIBODY AVAILABLE!
Anti-Ubiquitin, Lys63-Specific, clone Apu3 (catalogue # 05-1308)

This product is a native K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules. It can also be used to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs).
This product is formed by chemical ligation.

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.

In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Applicazioni

LC-MS Analysis
Mobile phase A = 1% CH3CN, 0.1% formic acid in water (MilliQ) and B = 1% water (milliQ) and 0.1% formic acid in
CH3CN. Phenomenex Kinetex C18, (2.1×50 mm, 2.6 μM); flow rate = 0.6 mL/min, runtime = 6 min, column T = 40°C.
Gradient: 5% to 95% B over 3.5 min. (See Figure 2)

Componenti

50 µg of Di-Ubiquitin (Lys63-linked) reconstituted in 2.5 µL DMSO and 56 µL of 0.02 M TRIS, 0.25 M NaCl, pH 7.5 to a final concentration of 50 µM.

Qualità

Western Blot Analysis:
0.5-2 μg/mL of anti-Ubiquitin, Lys63-Specific, clone Apu2 (Cat. # 05-1308) detected Lys63-linked Ubiquitin from 1 µg of Di-Ubiquitin (Lys63-linked) Recombinant Protein (Cat. # 17-10388). (See Figure 1)

Stoccaggio e stabilità

Store at -80°C. Avoid freeze/thaw cycles.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 188.6 °F - (refers to pure substance)
• Punto d’infiammabilità (°C): 87 °C - (refers to pure substance)