14-758-M

Ret (V804L) Protein, active, 10 µg

Active, N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804L mutation. For use in Kinase Assays.

• Codice UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 100; 300
• Ricombinante: expressed in baculovirus infected Sf21 cells
• PM: M_w 79.2 kDa
• Produttore/marchio commerciale: Upstate^®
• tecniche: activity assay: suitable (kinase)
• N° accesso NCBI: NM_000323
• N° accesso UniProt: P07949; P35546 (mouse)
• Informazioni sul gene: human ... RET(5979)

Descrizione

Descrizione generale

N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804L mutation.

Product Source: Expressed by baculovirus in Sf21 insect cells.

The V804L mutation has been identified in patients with familial medullary thyroid carcinoma (FMTC) (Lombardo F. et al., (2002), JCEM, 87, 1674-1680; Lesueur F. et al, (2005), JCEM, 90, 3454-3457).

Applicazioni

Research Category
Inflammation & Immunology

Azioni biochim/fisiol

Protein Target: Ret

Target Sub-Family: TK

Confezionamento

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-758M when ordering the 250 µg size.

Qualità

Routinely evaluated by phosphorylation of CHKtide.

Stato fisico

50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Glutathione agarose affinity chromatography

Stoccaggio e stabilità

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable