14-734-M

FGFR1 (V561M) Protein, active, 10 µg

Active, N-terminal GST-tagged, recombinant, human FGFR1 amino acids 456 -765 containing the V561 mutation. For use in Enzyme Assays.

• Sinonimo/i: Basic Fibroblast Growth Factor Receptor 1, c-fgr, CD331
• Codice UNSPSC: 12352200
• eCl@ss: 32160405
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in baculovirus infected Sf21 cells
• PM: M_w 62.3 kDa
• Produttore/marchio commerciale: Upstate^®
• tecniche: activity assay: suitable
• N° accesso NCBI: NM_015850.3; NM_023105.2; NM_023106.2; NM_023107.2; NM_023108.2; NM_023110.2; NM_023111.2
• N° accesso UniProt: P11362
• Informazioni sul gene: human ... FGFR1(2260)

Descrizione

Descrizione generale

N-terminal GST-tagged, recombinant, human FGFR1 amino acids 456 - 765 containing the V561 mutation. The coordinates for recombinant human FGFR1 were described in Homann et al. (2001). Blencke et al. (2004) suggested mutations to a conserved threonine residue at the ATP binding site would result in inhibitor resistance. The amino acid valine 561 was mutated to a methionine in FGFR1 which corresponded to previously reported mutations found in Abl (T315) and EGFR (T766) which had been shown to confer resistance to selective inhibitors. Assay data for FGFR1 V561M had shown that this mutation had conferred resistance to PP58 (pyrido [2,3-d] pyrimidine tyrosine kinase inhibitor) compared to that of the wild type.

Product Source: Recombinant human FGFR1 expressed by baculovirus in Sf21 cells.

Applicazioni

Research Category
Metabolism

Research Sub Category
Obesity

Metabolic Disorders

Azioni biochim/fisiol

Protein Target: FGFR1

Target Sub-Family: TK

Qualità

Routinely evaluated by phosphorylation of 500μM GGEEEEYFELVKKKK

Stato fisico

Glutathione-agarose

Presented as a frozen solution in 50mM Tris/HCl pH 7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Stoccaggio e stabilità

Stable for 6 months at -70°C from date of shipment. Upon first use, rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Altre note

Concentration: Please see lot specific Certificarte of Analysis.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Please see lot specific Certificarte of Analysis.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable