14-476-M
Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg
Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.
Sinonimo/i:
CDKN7, Cell division protein kinase 7, MO15, STK1, Serine/threonine-protein kinase 1
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About This Item
Codice UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Prodotti consigliati
Origine biologica
human
Livello qualitativo
Ricombinante
expressed in baculovirus infected Sf21 cells
Stato
liquid
Durata
1 yr
PM
Mw 37.8 kDa (cyclin H)
Mw 39.9 kDa (cdk7)
Mw 62.8 kDa (MAT1)
Produttore/marchio commerciale
Upstate®
tecniche
activity assay: suitable (kinase)
Solubilità
soluble
N° accesso NCBI
N° accesso UniProt
Temperatura di conservazione
−70°C
Informazioni sul gene
human ... CDK7(1022)
Descrizione generale
Research area: APOPTOSIS
Protein Target: CDK7/cyclinH/MAT1 Target Sub-Family: CMGC
Complex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag Product Source: All are expressed by baculovirus in Sf21 insect cells
Cdk-activating kinase (CAK) is a trimeric complex composed of cyclin-dependent kinase 7 (Cdk7), cyclin H, and RING finger protein Methionine Adenosyltransferase 1 (MAT1). Cyclin H activates Cdk7, while MAT1 plays a role in influencing the substrate specificity of the complex.
Protein Target: CDK7/cyclinH/MAT1 Target Sub-Family: CMGC
Complex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag Product Source: All are expressed by baculovirus in Sf21 insect cells
Cdk-activating kinase (CAK) is a trimeric complex composed of cyclin-dependent kinase 7 (Cdk7), cyclin H, and RING finger protein Methionine Adenosyltransferase 1 (MAT1). Cyclin H activates Cdk7, while MAT1 plays a role in influencing the substrate specificity of the complex.
Azioni biochim/fisiol
The Cdk7/cyclin H/Mat1 (CAK complex) plays a role in both cell cycle regulation and transcription. Within the complex, Cdk7 functions as the Cdk-activating kinase for cell cycle and as a part of the general transcription factor, Transcription Factor II H (TFIIH), for transcription process. Additionally, it also regulates gene expression by phosphorylating transcription factors such as estrogen receptor-α (ER), p53, retinoid receptors, androgen receptor, and estrogen receptor. Elevated expression levels of Cdk7, cyclin H, and MAT1 have been observed in breast cancer patients. Inhibition of cdk7 is associated with the development of colorectal cancer in humans.
Confezionamento
Also available in 250μg size, please inquire for pricing and availability.
Qualità
routinely evaluated by phosphorylation of cdk7 substrate peptide
Stato fisico
Ni2+/NTA-agarose
Stoccaggio e stabilità
1 year at -70°C
Altre note
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Tinggui Yin et al.
Molecular cancer therapeutics, 13(6), 1442-1456 (2014-04-02)
DNA-dependent RNA polymerase II (RNAP II) largest subunit RPB1 C-terminal domain (CTD) kinases, including CDK9, are serine/threonine kinases known to regulate transcriptional initiation and elongation by phosphorylating Ser 2, 5, and 7 residues on CTD. Given the reported dysregulation of
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