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126593

Sigma-Aldrich

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

Sinonimo/i:

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

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About This Item

Numero CAS:
Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.25

Descrizione

Merck USA index - 14, 8468

Saggio

≥98% (agarose gel electrophoresis)

Stato

solid

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze

Impurezze

≤3.0 ash
≤5.0% moisture

Colore

off-white

Solubilità

0.15 M sodium chloride: 10 mg/mL

Cationi in tracce

heavy metals: ≤10 ppm

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Descrizione generale

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment.
Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.

Applicazioni

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:
  • to block tissue sections of placenta in immunohistochemical (IHC) analysis
  • as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation
  • as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo

Azioni biochim/fisiol

Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs. It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Attenzione

Toxicity: Standard Handling (A)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter

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