1.25139

Resina Fractogel^® EMD SO₃ ⁻ (M)

RoboColumn Fractogel^® SO3 (M), volume 0.6 mL, prepacked column

• Sinonimo/i: RoboColumn Fractogel^® SO3 (M), 0.6 ml
• Codice UNSPSC: 41115711

Proprietà

• ligand: (Sulfoisobutyl)
• Materiali: polypropylene housing
• Nome Commerciale: Fractogel^®
• Forma fisica: prepacked column
• Parametri: ≤8 bar pressure; ~200 cm/hr max. flow rate
• column I.D. × L: 5 mm × 30 mm
• Volume: 0.6 mL
• Gruppo funzionale matrice: Methacrylate
• Dimens. media particelle: 40-90 μm
• Capacità: ~130 mL, resin binding capacity
• applicazioni: mAb; recombinant protein; vaccine development
• Tecnica di separazione: cation exchange
• Temperatura di conservazione: 15-25°C

Descrizione

Descrizione generale

5 x 30 mm

Caratteristiche e vantaggi

RoboColumn Fractogel^® SO3 (M), 0.6 ml enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Note legali

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany