1.15940

Crystal violet (C.I. 42555)

for microscopy Certistain^®

• Sinonimo/i: Crystal violet (C.I. 42555), Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B
• Formula empirica (notazione di Hill): C₂₅H₃₀ClN₃
• Numero CAS: 548-62-9
• Peso molecolare: 407.98
• Numero MDL: MFCD00011750
• Codice UNSPSC: 41116130
• Numero indice EU: 208-953-6

Proprietà

• Livello qualitativo: 400
• Stato: solid
• Potenza: 420 mg/kg LD₅₀, oral (Rat)
• Composizione: Dye, ≥88% spectrophotometric assay
• pH: 2.5-3.5 (20 °C, 10 g/L in H₂O)
• Punto di fusione: 189-194 °C
• Solubilità: 10 g/L
• Densità: 1.19 g/cm³ at 20 °C
• Densità bulk: 220‑400 kg/m³
• εmax: 2050-2450 at 589-594 nm in water at 0.002 g/L
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: 2-30°C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1; ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Descrizione

Descrizione generale

Crystal violet (C.I. 42555) for microscopy Certistain^®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The Certistain^® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Risultati analitici

Dye content (spectrophotometrically): ≥ 88%
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test

Note legali

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS07,GHS08,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H302 - H318 - H351 - H410
• Consigli di prudenza: P201 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Classi di pericolo: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3