1.03867
GranuCult® plus Deoxycholate Hydrogen sulfide Lactose Agar
acc. SAKAZAKI
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About This Item
Codice UNSPSC:
41106213
NACRES:
NA.74
Prodotti consigliati
Livello qualitativo
Forma fisica
powder
tecniche
microbiological culture: suitable
applicazioni
food and beverages
pharmaceutical
Compatibilità
nonselective for enterobacteriaceae
Descrizione generale
GranuCult® plus Deoxycholate Hydrogen sulfide Lactose (DHL) Agar is a nutrient rich medium to detect and isolate Gram-negative bacteria, from different materials. H2S production is indicated by a blackening of the colonies due to formation of iron sulfide. Although Proteus is H2S-positive, its colonies are not black.
Colonies of Proteus, Morganella, Rettgerella and Providencia are, however, surrounded by dark brown zones, which occur, because these species act on the phenylalanine of the peptone to produce phenylpyruvate, which forms an iron complex with iron(III) ions.
The sucrose content of the medium permits differentiation of weakly lactose-positive or lactose-negative, sucrose-positive species from sucrose- and lactose-negative Enterobacteriaceae.
The deoxycholate largely suppresses the growth of Gram-positive bacteria and prevents the swarming of Proteus species. This medium provides a rich nutrient base and contains a relatively low concentration of the inhibitor deoxycholate. These properties permit growth of even fastidious strains of Salmonella and Shigella. The colonies formed are considerably larger than those found on other selective culture media. Proteus, Morganella, Rettgerella and Providencia can be differentiated from Salmonella.
Neutral Red is a pH indicator and agar is the solidifying agent. .
Colonies of Proteus, Morganella, Rettgerella and Providencia are, however, surrounded by dark brown zones, which occur, because these species act on the phenylalanine of the peptone to produce phenylpyruvate, which forms an iron complex with iron(III) ions.
The sucrose content of the medium permits differentiation of weakly lactose-positive or lactose-negative, sucrose-positive species from sucrose- and lactose-negative Enterobacteriaceae.
The deoxycholate largely suppresses the growth of Gram-positive bacteria and prevents the swarming of Proteus species. This medium provides a rich nutrient base and contains a relatively low concentration of the inhibitor deoxycholate. These properties permit growth of even fastidious strains of Salmonella and Shigella. The colonies formed are considerably larger than those found on other selective culture media. Proteus, Morganella, Rettgerella and Providencia can be differentiated from Salmonella.
Neutral Red is a pH indicator and agar is the solidifying agent. .
Applicazioni
GranuCult® plus Deoxycholate Hydrogen sulfide Lactose (DHL) Agar is used to isolate medium to detect and isolate Gram-negative bacteria from different materials. The gram-positive bacterial growth is inhibited by the medium properties that accompany the test sample.
Altre note
DHL (Deoxycholate Hydrogen sulfide Lactose) agar acc. to SAKAZAKI is also known as Sakazaki DHL agar.
Nota a piè di pagina
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Note legali
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
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