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Merck
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Documenti fondamentali

05-484

Sigma-Aldrich

Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3

clone 20G3, Upstate®, from mouse

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

20G3, monoclonal

Reattività contro le specie

human, mouse

Produttore/marchio commerciale

Upstate®

tecniche

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1κ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

phosphorylation (non-phospho-Tyr1173)

Informazioni sul gene

human ... EGFR(1956)
mouse ... Egfr(13649)

Specificità

The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.

Immunogeno

peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor

Applicazioni

Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Qualità

routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells

Descrizione del bersaglio

175kDa

Linkage

Replaces: 04-337; 04-341

Stato fisico

100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Format: Purified
Thiophilic adsoption and size exclusion chromatography

Stoccaggio e stabilità

2 years at -20°C

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

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Gang Chen et al.
Biological procedures online, 13, 1-1 (2011-03-04)
Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was estimated by RT-qPCR
Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors.
Sorkin, A, et al.
The Journal of Biological Chemistry, 267, 8672-8678 (1992)
Alteration of epidermal growth factor receptor activity by mutation of its primary carboxyl-terminal site of tyrosine self-phosphorylation.
Bertics, P J, et al.
The Journal of Biological Chemistry, 263, 3610-3617 (1988)
Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice.
Siggs, OM; Grieve, A; Xu, H; Bambrough, P; Christova, Y; Freeman, M
Biology Open null

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