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Merck
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Key Documents

05-363

Sigma-Aldrich

Anti-Cyclin E Antibody, clone HE12

clone HE12, Upstate®, from mouse

Sinonimo/i:

Anti-CCNE, Anti-pCCNE1

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

HE12, monoclonal

Reattività contro le specie

human

Confezionamento

antibody small pack of 25 μg

Produttore/marchio commerciale

Upstate®

tecniche

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

NM_001238
NM_057182

N° accesso UniProt

P24864

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CCNE1(898)

Descrizione generale

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Specificità

human p52cyclin E

Immunogeno

Recombinant human cyclin E

Applicazioni

Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC.

Qualità

routinely evaluated in immunoblot on human A431 lysate

Descrizione del bersaglio

52 kDa

Linkage

Replaces: 04-223

Stato fisico

Format: Purified

Stoccaggio e stabilità

2 years at -20°C or 6 months at 4°C

Risultati analitici

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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S M Keenan et al.
The Journal of biological chemistry, 276(25), 22404-22409 (2001-04-17)
Activation of cyclin-dependent kinase 2 (CDK2)-cyclin E in the late G(1) phase of the cell cycle is important for transit into S phase. In Chinese hamster embryonic fibroblasts (IIC9) phosphatidylinositol 3-kinase and ERK regulate alpha-thrombin-induced G(1) transit by their effects
Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells.
Maimets, T; Neganova, I; Armstrong, L; Lako, M
Oncogene null
Hae-June Lee et al.
Radiation research, 170(5), 579-590 (2008-10-31)
The aim of this work was to identify specific genes involved in rat mammary tumors induced by dimethylbenz(a)anthracene (DMBA) or radiation. More TUNEL- and PCNA-positive cells were present in mammary tumors induced by radiation than in tumors induced by DMBA
Inês Gomes et al.
Oncotarget, 11(19), 1714-1728 (2020-06-02)
The role of RANKL-RANK pathway in progesterone-driven mammary carcinogenesis and triple negative breast cancer tumorigenesis has been well characterized. However, and despite evidences of the existence of RANK-positive hormone receptor (HR)-positive breast tumors, the implication of RANK expression in HR-positive
P Saha et al.
Molecular and cellular biology, 17(8), 4338-4345 (1997-08-01)
Cdc25A, a phosphatase essential for G1-S transition, associates with, dephosphorylates, and activates the cell cycle kinase cyclin E-cdk2. p21CIP1 and p27 are cyclin-dependent kinase (cdk) inhibitors induced by growth-suppressive signals such as p53 and transforming growth factor beta (TGF-beta). We
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