05-1339
Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304
clone CMA304, from mouse
Sinonimo/i:
H3K4me3, Histone H3 (tri methyl K4), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
CMA304, monoclonal
Reattività contro le specie
human, vertebrates
tecniche
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
multiplexing: suitable
western blot: suitable
Isotipo
IgG1κ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
trimethylation (Lys4)
Informazioni sul gene
human ... H3C1(8350)
Descrizione generale
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H3 (Lys4) is is found at the 5′ end of active genes and contributes to transcriptional activation by recruiting chromatin-remodelling enzymes. Accordingly, the inactive X chromosome is largely devoid of this mark.
Specificità
Broad species cross-reactivity is expected, based on sequence homology.
This antibody specifically recognizes Histone H3 trimethylated at Lys4. The antibody binding specificity allows for modifications of Lys91.
Immunogeno
Epitope: Trimethylated Lys4
Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, trimethylated on Lys4, conjugated to KLH.
Applicazioni
Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304 is a mouse monoclonal antibody for detection of Trimethyl Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in ELISA, ICC, IP, WB, Mplex, DB.
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
Multiplexing:
This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex® assay.
This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
Multiplexing:
This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex® assay.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Qualità
Western Blot Analysis:
0.5 - 2 μg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
0.5 - 2 μg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
Descrizione del bersaglio
approx. 17 kDa
Linkage
Replaces: 04-791
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Risultati analitici
Control
HeLa Acid extract lysate
HeLa Acid extract lysate
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
Luminex is a registered trademark of Luminex Corp
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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