04-841

Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

culture supernatant, clone A9W4, from rabbit

• Sinonimo/i: RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: culture supernatant
• Tipo di anticorpo: primary antibodies
• Clone: A9W4, monoclonal
• Reattività contro le specie: rat, human, mouse, bovine
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NM_013022
• N° accesso UniProt: Q62868
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: rat ... Rock2(25537)

Descrizione

Descrizione generale

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

Specificità

Predicted to cross-react with mouse and bovine based on sequence homology.

Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.

Immunogeno

6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.

Applicazioni

Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.

Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Qualità

Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.

Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.

Descrizione del bersaglio

150 kDa

Linkage

Replaces: 05-841

Stato fisico

Cultured supernatant containing 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
RIPA lysates from PC-12 cells

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable