04-1557
Anti-SIRT1 Antibody, clone 10E4
clone 10-E-4, from mouse
Sinonimo/i:
SIR2-like protein 1, sir2-like 1, sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtuin type 1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
10-E-4, monoclonal
Reattività contro le specie
mouse, human
Confezionamento
antibody small pack of 25 μL
tecniche
ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
ambient
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... SIRT1(23411)
Descrizione generale
Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism′s response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.
Specificità
This antibody recognizes SIRT1.
Immunogeno
Epitope: Unknown
His-tagged recombinant protein corresponding to human SIRT1.
Applicazioni
Does not react in mouse in IHC. Mouse sequence is 100% identical to immunogen, so it is predicted to work in western blotting.
Immunoprecipitation: Reported by independent researcher using representative lot.
Chromatin Immunopreiciptiation: Reported by independent researcher using representative lot.
Immunohistochemistry: Reported by independent researcher using representative lot.
Chromatin Immunopreiciptiation: Reported by independent researcher using representative lot.
Immunohistochemistry: Reported by independent researcher using representative lot.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC, ChIP to detect SIRT1 also known as sirtuin (silent mating type information regulation 2 homolog) 1, sirtuin 1.
Qualità
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected SIRT1 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected SIRT1 on 10 µg of HeLa cell lysate.
Descrizione del bersaglio
~ 110 kDa
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Risultati analitici
Control
HeLa cell lysate
HeLa cell lysate
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
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The Journal of biological chemistry, 284(16), 10361-10366 (2009-02-17)
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SIRT1 is a principle class III histone deacetylase which exhibits versatile functions in stress response, development, and pathological processes including cancer. Although SIRT1 deacetylates a wide range of nuclear and cytoplasmic proteins, its subcellular localization in cancer cells has been
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