906476
SLAM-Aβ-13CHD2 Methyl Labeling Kit
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About This Item
tecniche
bio NMR: suitable
Livello qualitativo
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Descrizione generale
SLAM-Aβ-13CHD2 kit has 13CHD2 isotopomer precursor and contains protocol instructions for creation of isotopically-labeled proteins.
Applicazioni
For solid-state NMR applications and dynamic studies.
For regio- and/or stereo- specific isotope labelilng of Isoleucine, Leucine, Valine, and Alanine with 13CHD2 isotopomers.
For regio- and/or stereo- specific isotope labelilng of Isoleucine, Leucine, Valine, and Alanine with 13CHD2 isotopomers.
SLAM-Aβ-13CHD2 kit is used for labeling alanine precursor with 13CHD2 isotopomer. This kit has been tested with protein isotopic labeling in E. coli. It can be employed for the study of side chain dynamics of protein in the solid-state NMR spectroscopy.
Confezionamento
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Codice della classe di stoccaggio
11 - Combustible Solids
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Journal of the American Chemical Society, 127(32), 11208-11209 (2005-08-11)
We show in this communication that dynamic information for uniformly 2H,13C,15N isotopically enriched, crystalline proteins can be obtained by MAS solid-state NMR spectroscopy. The experiments make use of the deuterium quadrupolar tensor, which is the dominant interaction mechanism. Dynamic properties
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that
Articoli
Methyl Isotope-Labeling of Proteins from NMR-Bio
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
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