532290

2-Chlorotrityl chloride, polymer-bound

200-400 mesh, extent of labeling: 1.0-1.5 mmol/g Cl loading, 1 % cross-linked

• Sinonimo/i: 2,α-Dichlorobenzhydryl-polystyrene crosslinked with divinylbenzene
• Numero CAS: 42074-68-0
• Beilstein: 1983914
• Numero MDL: MFCD00040399
• Codice UNSPSC: 12352107
• ID PubChem: 24877876
• NACRES: NA.22

Proprietà

• Crosslinking: 1 % cross-linked
• Impiego in reazioni chimiche: reaction type: Fmoc solid-phase peptide synthesis
• Grado di funzionalizzazione: 1.0-1.5 mmol/g Cl loading
• Dimensione particelle: 200-400 mesh
• applicazioni: peptide synthesis
• Stringa SMILE: Clc1ccccc1C(Cl)(c2ccccc2)c3ccccc3
• InChI: 1S/C19H14Cl2/c20-18-14-8-7-13-17(18)19(21,15-9-3-1-4-10-15)16-11-5-2-6-12-16/h1-14H; JFLSOKIMYBSASW-UHFFFAOYSA-N

Descrizione

Applicazioni

• Acid labile resin used in Fmoc-based solid phase peptide synthesis.
• Mild acidic cleavage conditions lead to the release of the peptide acid where fully protected peptides can be released if desired.
• 2-Chlorotrityl chloride resins prevent racemization of the first amino acid and are thus very useful when racemic mixtures are forming (common with residues such as His or Cys).
• This resin also prevents diketopiperazide formation, which can be an issue with proline C-terminal peptide sequences.

Use:

• Attachment of the first amino acid residue is effected by stirring the resin, the protected amino acid, and excess diisopropylethylamine (DIEA) in dichloromethane.
• Cleavage of the final protected peptide fragment is achieved under very mild conditions using either acetic acid/trifluoroethanol (TFE)/dichloromethane (1:1:8; v/v/v), hexafluoroisopropanol (HFIP)/dichloromethane (1:4; v/v) or simply 0.5% trifluoroacetic acid/dichloromethane (v/v).
• Higher concentrations of TFA can be used if retention of peptide side chaing protecting groups is unimportant. Note that trityl chloride is moisture-sensitive, and, therefore, should be stored and handled appropriately.
• If the resin becomes deactivated, treatment with acetyl chloride or SOCl₂ in toluene before use is recommended to restore its activity.

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H290,H315,H319,H335
• Consigli di prudenza: P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves