Accéder au contenu
Merck

A20-deficient mast cells exacerbate inflammatory responses in vivo.

PLoS biology (2014-01-24)
Klaus Heger, Kaat Fierens, J Christoph Vahl, Attila Aszodi, Katrin Peschke, Dominik Schenten, Hamida Hammad, Rudi Beyaert, Dieter Saur, Geert van Loo, Axel Roers, Bart N Lambrecht, Mirjam Kool, Marc Schmidt-Supprian
RÉSUMÉ

Mast cells are implicated in the pathogenesis of inflammatory and autoimmune diseases. However, this notion based on studies in mast cell-deficient mice is controversial. We therefore established an in vivo model for hyperactive mast cells by specifically ablating the NF-κB negative feedback regulator A20. While A20 deficiency did not affect mast cell degranulation, it resulted in amplified pro-inflammatory responses downstream of IgE/FcεRI, TLRs, IL-1R, and IL-33R. As a consequence house dust mite- and IL-33-driven lung inflammation, late phase cutaneous anaphylaxis, and collagen-induced arthritis were aggravated, in contrast to experimental autoimmune encephalomyelitis and immediate anaphylaxis. Our results provide in vivo evidence that hyperactive mast cells can exacerbate inflammatory disorders and define diseases that might benefit from therapeutic intervention with mast cell function.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
4-Hydroxytamoxifène, ≥70% Z isomer (remainder primarily E-isomer)
Sigma-Aldrich
2-mercaptoéthanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Sigma-Aldrich
2-mercaptoéthanol, ≥99.0%
Sigma-Aldrich
Chlorure de calcium solution, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
Iodure de propidium, ≥94.0% (HPLC)
Sigma-Aldrich
Calcium chloride, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
Sigma-Aldrich
2-mercaptoéthanol, BioUltra, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
Anticorps anti-dinitrophényle monoclonal antibody produced in mouse, IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
1-Fluoro-2,4-dinitrobenzene, ≥99%
Sigma-Aldrich
Aluminum hydroxide, reagent grade
Sigma-Aldrich
Calcium chloride, anhydrous, powder, 99.99% trace metals basis
Sigma-Aldrich
1-Fluoro-2,4-dinitrobenzene, purum p.a., ≥98.0% (GC)
Sigma-Aldrich
Calcium chloride, AnhydroBeads, −10 mesh, ≥99.9% trace metals basis
Sigma-Aldrich
Iodure de propidium solution
Sigma-Aldrich
Chlorure de calcium dihydrate
Sigma-Aldrich
Calcium chloride, AnhydroBeads, −10 mesh, ≥99.99% trace metals basis
Sigma-Aldrich
Iodure de propidium, ≥94% (HPLC)
Supelco
2-mercaptoéthanol, for HPLC derivatization, LiChropur, ≥99.0% (GC)
Supelco
Chlorure de calcium solution, 0.1 M Ca, analytical standard (for ion-selective electrodes)
Supelco
4-Hydroxytamoxifène, analytical standard, (E) and (Z) isomers (50:50)
Supelco
1-Fluoro-2,4-dinitrobenzene, for HPLC derivatization, LiChropur, ≥99.0% (GC)
Supelco
4-Hydroxytamoxifène, (E) and (Z) isomers (50:50), analytical standard