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Y0396

Sigma-Aldrich

Anti-YB-1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-BP-8, Anti-CSDA2, Anti-CSDB, Anti-DBPB, Anti-MDR-NF1, Anti-NSEP1, Anti-Y BOX binding protein, Anti-YBOX1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~50 kDa

Espèces réactives

rat, human, mouse

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells
western blot: 0.5-1 μg/mL using PC12 cell lysates

Numéro d'accès UniProt

P67809

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... YBX1(4904)
mouse ... Ybx1(22608)
rat ... Ybx1(500538)

Description générale

Y-box-binding protein (YB-1) also known as nuclease-sensitive element-binding protein 1 (NSEP1) is a member of the Cold-Sock Domain (CSD) containing proteins. It is composed of three domains: the N-terminal which is thought to function as a trans-activation regulatory domain, a highly conserved nucleic acid binding domain (the CSD), and C-terminal domain, involved in protein-protein interactions.

Spécificité

Anti-YB-1 (C-terminal) antibody is specific for YB-1 in rats, mice and humans. The immunizing peptide specifically inhibits the detection of the YB-1 band by immunoblotting.

Immunogène

synthetic peptide corresponding to amino acids 307-324 of human YB-1, conjugated to KLH. The corresponding sequence is identical in rat and mouse YB-1 proteins.

Application

Anti-YB-1 (C-terminal) antibody has been used in
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence
  • double-immunostaining

Actions biochimiques/physiologiques

Y-box-binding protein (YB-1) is shown to bind to the Y-box promoter element to regulate gene expression. It plays an important role in a wide variety of cellular functions such as transcriptional and translation regulation, DNA repair, drug resistance, and stress responses.

Forme physique

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Kimitoshi Kohno et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 25(7), 691-698 (2003-06-20)
The Y-box-binding protein (YB-1) represents the most evolutionary conserved nucleic-acid-binding protein currently known. YB-1 is a member of the cold-shock domain (CSD) protein superfamily. It performs a wide variety of cellular functions, including transcriptional regulation, translational regulation, DNA repair, drug
Daniel M Breitkopf et al.
Kidney international, 97(2), 289-303 (2019-12-29)
Systemic lupus erythematosus (SLE) is an autoimmune disease and lupus nephritis is a major risk factor for morbidity and mortality. Notch-3 signaling induced by membrane-bound or soluble ligands such as YB-1 constitutes an evolutionarily conserved pathway that determines major decisions
K Matsumoto et al.
Trends in cell biology, 8(8), 318-323 (1998-08-15)
Y-box proteins are multifunctional regulators of gene expression. In somatic cells, they have the capacity to exert positive and negative effects on both transcription and translation. In Xenopus oocytes, they help to mask maternal mRNA and couple the transcription of
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts
Wei W J, et al.
Nucleic Acids Research, 40(17), 8622-8636 (2012)
N V Bobkova et al.
PloS one, 10(9), e0138867-e0138867 (2015-09-24)
The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously
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