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5006

Sigma-Aldrich

PureCol™

Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

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About This Item

Numéro CE :
232-697-4
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

Niveau de qualité

100

Stérilité

sterile-filtered

Pureté

99.9%

Forme

lyophilized

Conditionnement

pkg of 15 mg

Technique(s)

cell culture | mammalian: suitable

Spécificité de la liaison

Peptide Source: Elastin

Peptide Source: Fibrinogen

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Application

PureCol™ has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Notes préparatoires

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Informations légales

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Rebecca Long Heise et al.
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