X2252

Xanthine Oxidase microbial

lyophilized powder, ≥7 units/mg solid

• Synonyme(s) : Schardingerenzyme, XO, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
• Numéro CAS: 9002-17-9
• Numéro de classification (Commission des enzymes): 1.17.3.2 (BRENDA, IUBMB)
• Numéro CE : 232-657-6
• Numéro MDL: MFCD00082145
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: microbial
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥7 units/mg solid
• Poids mol.: ~160 kDa
• Conditions de stockage: (Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized; persons)
• Technique(s): inhibition assay: suitable
• Couleur: brown
• pH optimal: 7.5-8.0
• Numéro d'accès UniProt: Q46799; Q46800; Q46801
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)

Description

Description générale

Research area: Cell Cycle

Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.

Application

Xanthine Oxidase microbial has been used:

• to determine in vitro xanthine oxidase activity
• as a source to generate superoxide anions during superoxide dismutase assay
• as a component of the reagent mix in colorimetric paper-based device to detect allopurinol in traditional medicine by enzymatic reaction

Actions biochimiques/physiologiques

Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism. This enzyme is also implicated in caffeine metabolism. XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid. Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.

Also contains proteolytic activity.

Propriétés physiques

Inhibitors: Reducing agents, Hg⁺⁺, Ag⁺,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65^oC
pH Stability: pH 6.5 - 9.0 (25^oC, 15hr)
Thermal stability: below 55^oC (pH 8.0, 30min)

Définition de l'unité

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Forme physique

Lyophilized powder containing BSA and sodium glutamate as stabilizers

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P304 + P340 + P312 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)