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Merck
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Principaux documents

T7575

Sigma-Aldrich

Trypsin Singles, Proteomics Grade

Synonyme(s) :

Proteomics grade, Trypsin, TPCK treated

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.56

Qualité

Proteomics Grade

Niveau de qualité

Forme

ready-to-use solution

Activité spécifique

≥10,000 BAEE units/mg protein

Poids mol.

average mol wt 23.29 kDa

pH optimal

~8.0

Température de stockage

2-8°C

Description générale

Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.

Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.

Application

Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry). It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.

Caractéristiques et avantages

  • Convenient, single-use 1 μg size
  • Eliminates repetitive pipetting
  • Reductively methylated to minimize autolytic activity
  • TPCK treated to quench chymotryptic activity
  • Extensively purified

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • Trypsin Solubilization Reagent

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

FlameHealth hazardExclamation mark

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

3 - Flammable liquids


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Consulter la Bibliothèque de documents

Arun K Ramani et al.
Molecular systems biology, 4, 180-180 (2008-04-17)
The human protein interaction network will offer global insights into the molecular organization of cells and provide a framework for modeling human disease, but the network's large scale demands new approaches. We report a set of 7000 physical associations among
Daniel W Udwary et al.
Applied and environmental microbiology, 77(11), 3617-3625 (2011-04-19)
Bacteria of the genus Frankia are mycelium-forming actinomycetes that are found as nitrogen-fixing facultative symbionts of actinorhizal plants. Although soil-dwelling actinomycetes are well-known producers of bioactive compounds, the genus Frankia has largely gone uninvestigated for this potential. Bioinformatic analysis of
Michael Costanzo et al.
Science (New York, N.Y.), 327(5964), 425-431 (2010-01-23)
A genome-scale genetic interaction map was constructed by examining 5.4 million gene-gene pairs for synthetic genetic interactions, generating quantitative genetic interaction profiles for approximately 75% of all genes in the budding yeast, Saccharomyces cerevisiae. A network based on genetic interaction
Tiziana Cabras et al.
Molecular & cellular proteomics : MCP, 9(10), 2099-2108 (2010-06-30)
The acidic soluble fraction of whole saliva of type 1 diabetic children was analyzed by reversed phase (RP)(1)-HPLC-ESI-MS and compared with that of sex- and age-matched control subjects. Salivary acidic proline-rich phosphoproteins (aPRP), histatins, α-defensins, salivary cystatins, statherin, proline-rich peptide
Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry.
Tripkovic T, et al.
Talanta, 113, 49-61 (2013)

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