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Key Documents

T4528

Sigma-Aldrich

Thioglucosidase from Sinapis alba (white mustard) seed

≥100 units/g solid

Synonyme(s) :

Glucosinolase, Thioglucoside glucohydrolase

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

plant seeds (Sinapis alba)

Niveau de qualité

Forme

solid

Activité spécifique

≥100 units/g solid

Température de stockage

−20°C

Description générale

Thioglucosidase, also called as myrosinase, is present in the myrosin cells that do not contain glucosinolates. This enzyme is obtained from several plant sources, such as Lepidium sativum, L. Sinapis alba and Brassica napus.

Application

Thioglucosidase from Sinapis alba (white mustard) seeds has been used as a standard to quantify myrosinase activity and in column glucosinolate analysis of plant samples.
Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content. Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana.

Actions biochimiques/physiologiques

Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits.
Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties.

Définition de l'unité

One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Palatability of Thlaspi caerulescens for snails: influence of zinc and glucosinolates
Noret N, et al.
The New phytologist, 165(3), 763-772 (2005)
Bioavailability of Isothiocyanates From Broccoli Sprouts in Protein, Lipid, and Fiber Gels
Oliviero T, et al.
Molecular Nutrition And Food Research, 62(18), 1700837-1700837 (2018)
Si-Jun Zheng et al.
Journal of chemical ecology, 37(8), 818-829 (2011-06-22)
The jasmonic acid (JA) signaling pathway and defensive secondary metabolites such as glucosinolates are generally considered to play central roles in the defense of brassicaceous plants against herbivorous insects. To determine the function of specific plant genes in plant-insect interactions
Elena Peñas et al.
Journal of agricultural and food chemistry, 59(8), 3772-3779 (2011-03-19)
The influence of two Spanish growing locations with well-differentiated climatic conditions (northern and eastern areas) on the main bioactive compounds, glucosinolates (GLS), total phenolic compounds (TPC), and vitamin C, as well as myrosinase activity and antioxidant capacity in five white
Chemical defence and toxins of plants
Yamane H, et al.
Journal of Hospital Infection (2010)

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