SAB4200483

Anti-JAK2 antibody, Mouse monoclonal

clone 10.1.5, purified from hybridoma cell culture

• Synonyme(s) : Anti-JAK-2, Anti-JTK10, Anti-Janus kinase 2, Monoclonal Anti-JAK2 antibody produced in mouse
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: 10.1.5, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~125 kDa
• Espèces réactives: bovine, human
• Concentration: ~1.0 mg/mL
• Technique(s): indirect immunofluorescence: 2.5-5.0 μg/mL using MCF7 cells.; western blot: 2.0-4.0 μg/mL using TF1 total cell extracts.
• Numéro d'accès UniProt: O60674
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... JAK2(3717)

Description

Description générale

Monoclonal Anti-JAK2 (mouse IgG1 isotype) is derived from the hybridoma 10.1.5 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. The Janus kinases (Jak) family of proteins includes four members: Jak1, Jak2, Jak3 and the tyrosine kinase 2 (Tyk2). Jak2 is ubiquitously expressed. It is also expressed in normal and neoplastic mammary epithelial cells.

Immunogène

synthetic peptide corresponding to an internal sequence of human JAK2 (GeneID 3717), conjugated to KLH.

Application

Monoclonal Anti-JAK2 antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• flow cytometry

Actions biochimiques/physiologiques

Janus kinases engage in interactions with the intracellular tails of several cytokine receptors, which result in the activation of members of the signal transducer and activator of transcription (STAT) family of transcription factors. The latter bind specific cognate sequences at the promoter region of genes that regulate proliferation and differentiation. JAK activation plays an important role in cell proliferation, differentiation, migration and apoptosis. A member of this family, JAK2, has been found to induce signaling regulating gene expression of various pro-survival and anti-apoptotic molecules. Furthermore, inhibition of JAK2 signaling triggered cell death in human cancer cells. These findings indicate JAK2 inhibition as a potential therapeutic approach for various cancers.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable