09-275

Anti-phospho-JAK2 (Tyr1007/1008) Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: pig, mouse
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), human (based on 100% sequence homology), chicken (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology), porcine (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_004963
• Numéro d'accès UniProt: O60674
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pTyr1007/pTyr1008)
• Informations sur le gène: chicken ... Jak2(374199) ; frog ... Jak2(100036599) ; human ... JAK2(3717) ; mouse ... Jak2(16452) ; pig ... Jak2(397201) ; rat ... Jak2(24514)

Description

Description générale

Janus kinase 2 (JAK2) is a ubiquitous non-receptor tyrosine kinase that interacts with multiple cell-surface receptors and non-receptor proteins to affect a range of cellular processes including immunity, cell growth, development, and differentiation. Among JAK2’s receptor partners are the prolactin, growth hormone, thrombopoitin, and INF (α and β) receptors. JAK2 plays an important role in connecting the signaling pathways of these receptors to the activation of target genes. Upon ligand binding these receptors activate JAK2 inducing JAK2 autophosphorylation; This activated JAK2 in turn phosphorylates the cytoplasmic domains of these receptors providing a binding site for STAT transcription factors which are also phosphorylated by JAK2 leading to STAT protein dimerization and translocation to the nucleus to effect gene transcription. Aberrations in JAK2 expression and function may contribute to myelofibrosis and myelogenous leukemia.

Spécificité

This antibody recognizes JAK2 phosphorylated at (Tyr1007/Tyr1008).

Immunogène

Epitope: Phosphorylated (Tyr1007/Tyr1008)

KLH-conjugated linear peptide corresponding to human JAK2 phosphorylated at (Tyr1007/Tyr1008).

Application

Anti-phospho-JAK2 (Tyr1007/1008) Antibody is an antibody against phospho-JAK2 (Tyr1007/1008) for use in WB.

Research Category
Signaling

Qualité

Evaluated by Western Blot in LPS/IFN gamma untreated and treated RAW264.7 cell lysates.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected JAK2 on 10 µg of LPS/IFN gamma untreated and treated RAW264.7 cell lysates.

Description de la cible

~130 kDa observed. Uncharacterized bands appear at ~48, 55, 65, and 100 kDa in some lysates.

Liaison

Replaces: 04-1098

Forme physique

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
LPS/IFN gamma untreated and treated RAW264.7 cell lysates

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable