S3401

Sample Buffer, Laemmli 2× Concentrate

• Synonyme(s) : SDS Sample Buffer
• Code UNSPSC : 41105323
• Nomenclature NACRES : NA.25

Propriétés

• Conditionnement: vial of 6 mL
• Niveau de qualité: 200
• Température de stockage: −20°C

Description

Application

Sample Buffer, Laemmli 2× concentrate has been used as a sample buffer for denaturing and loading of protein samples in SDS-PAGE.

Actions biochimiques/physiologiques

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.

Autres remarques

Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.

Quantité

Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.

Informations sur la sécurité

• Pictogrammes: GHS05,GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H302 - H311 - H315 - H317 - H318 - H361d - H373 - H412
• Conseils de prudence: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• Code de la classe de stockage: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter