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RAB0436

Sigma-Aldrich

Human Osteopontin ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonyme(s) :

OPN

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type serum
sample type plasma
sample type urine
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 74.07-18000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... SPP1(6696)

Catégories apparentées

Description générale

The Human OPN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human OPN in serum, plasma, cell culture supernatants and urine.

Immunogène

Recombinant Human OPN

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Osteopontin ELISA Kit has been used in the quantification of serum osteopontin (OPN) in human blood samples.

Actions biochimiques/physiologiques

Osteopontin (OPN) is a secreted glycoprotein and is assumed to be associated with various cellular processes such as adhesion, migration, and biomineralization in diverse tissues. Osteopontin acts as a ligand to αvβ3 integrin and possibly other receptors. In addition to its involvement in mineralized matrix formation, osteopontin can also function as a cytokine that stimulates the release of IFNγ (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin has proinflammatory functions and is associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and retards ectopic calcification by inhibiting the formation of hydroxyapatite. OPN is thought to prevent renal stone formation by suppressing nucleation, growth and aggregation of calcium oxalate crystals.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)FDS
  • RABSTOP3ELISA Stop Solution (Item I)FDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Can Osteopontin Be Considered a Biomarker for Endometriosis?
Albu D R, et al.
Revista de Chimie (Bucharest, Romania), 13(3), 1-1 (2017)
Octavio Gamaliel Aztatzi-Aguilar et al.
PloS one, 16(3), e0249057-e0249057 (2021-03-26)
Osteopontin (OPN) is a protein involved in inflammatory illnesses such as fibrosis and cancer; its overexpression in cardiovascular diseases promotes the biomineralization of blood vessels and other soft tissues. Moreover, there is an active component of oxidative stress related with
Post-translational modification and proteolytic processing of urinary osteopontin.
Christensen B, et al.
The Biochemical Journal, 411, 53-61 (2008)
The adhesive and migratory effects of osteopontin are mediated via distinct cell surface integrins. Role of alpha v beta 3 in smooth muscle cell migration to osteopontin in vitro.
Liaw L, et al.
The Journal of Clinical Investigation, 95, 713-724 (1995)
The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.
Chabas D, et al.
Science, 294, 1731-1735 (2001)
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