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Sigma-Aldrich

SYBR® Green Quantitative RT-qPCR Kit

One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase

Synonyme(s) :

one step rt qPCR, sybr green qPCR

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Utilisation

sufficient for 250 reactions

Caractéristiques

dNTPs included
hotstart

Technique(s)

RT-qPCR: suitable

Couleur

colorless

Entrée

purified RNA

Compatibilité

ABI 5700
 ABI 7000
 ABI 7300
 ABI 7500 Fast
 ABI 7500
 ABI 7700
 ABI 7900 HT
ABI 7900 HT Fast
 ABI 7900
 ABI StepOne
 ABI StepOnePlus
 ABI ViiA 7
 Bio-Rad CFX384
 Bio-Rad CFX96
 Bio-Rad MJ Chromo4
 Bio-Rad MJ Opticon 2
 Bio-Rad MJ Opticon
 Bio-Rad MiniOpticon
 Bio-Rad MyiQ
 Bio-Rad iCycler iQ
 Bio-Rad iQ5
 Cepheid SmartCycler
 Eppendorf® Mastercycler ep realplex2 s
 Eppendorf® Mastercycler ep realplex
 Illumina Eco qPCR
 Qiagen Corbett Rotor-Gene 3000
 Qiagen Corbett Rotor-Gene 6000
 Qiagen Corbett Rotor-Gene Q
 Roche LightCycler 480
 Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000

Méthode de détection

SYBR® Green

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

The SYBR® Green Quantitative RT-PCR Kit combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), JumpStart™ Taq DNA polymerase, and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Application

SYBR® Green Quantitative RT-qPCR Kit has been used in a 1-step reverse transcription polymerase chain reaction (RT-PCR) assay:
  • to detect specific genetic clusters of genogroup I and II noroviruses
  • for chikungunya viral (CHIKV) RNA quantification
  • to detect mRNA expression levels
  • for amplification of total RNA extracted from human umbilical vein endothelial cells (HUVECs) and prostate cancer cells

Caractéristiques et avantages

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced preparation time and the risk of contamination from multiple pipetting steps
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
  • SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
  • Broad instrument compatibility
  • Includes a separate ROX reference dye vial for reaction normalization

Conditionnement

1 kit sufficient for 100 reactions at 50 μL each

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Composants de kit seuls

Réf. du produit
Description
  • SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 includedFDS
  • M878725 mM MgCl2 1.5 mL/vialFDS
  • R4526Reference Dye for Quantitative PCR, 100 ×, solution .3 mL/vialFDS

Pictogrammes

CorrosionExclamation markEnvironment
GHS05,GHS07,GHS09

Mention d'avertissement

Danger

Mentions de danger

H314 - H317 - H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

Code de la classe de stockage

8A - Combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Carmela R Balistreri et al.
Scientific reports, 9(1), 11028-11028 (2019-08-01)
Bicuspid aortic valve (BAV) disease is recognized to be a syndrome with a complex and multifaceted pathophysiology. Its progression is modulated by diverse evolutionary conserved pathways, such as Notch-1 pathway. Emerging evidence is also highlighting the key role of TLR4
Pei Jin Lim et al.
PLoS neglected tropical diseases, 8(2), e2661-e2661 (2014-03-04)
Chikungunya virus (CHIKV) has resulted in several outbreaks in the past six decades. The clinical symptoms of Chikungunya infection include fever, skin rash, arthralgia, and an increasing incidence of encephalitis. The re-emergence of CHIKV with more severe pathogenesis highlights its
Gary P Richards et al.
Applied and environmental microbiology, 70(12), 7179-7184 (2004-12-03)
Genogroup I noroviruses from five genetic clusters and genogroup II noroviruses from eight genetic clusters were detected in stool extracts using degenerate primers and single-tube, real-time reverse transcription-PCR (RT-PCR) with SYBR Green detection. Two degenerate primer sets, designated MON 431-433
Phui San Ho et al.
Virology journal, 7, 13-13 (2010-01-23)
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus and one of the prevalent re-emerging arbovirus in tropical and subtropical regions of Asia, Africa, and Central and South America. It produces a spectrum of illness ranging from inapparent infection to moderate febrile
Caiyun Shan et al.
Molecular medicine reports, 17(4), 5300-5305 (2018-02-03)
Stroke is the most common cause of mortality worldwide. Post-stroke angiogenesis is of great significance to the treatment of strokes. The aim of the present study was to investigate the mechanism underlying the angiogenesis-promoting effect of microRNA‑126 (miR‑126)‑associated signaling pathways
Afficher tous les articles scientifiques apparentés

Articles

Quantitative RT-PCR

Sigma’s RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression

Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

PCR Master Mix

A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.

Protocoles

Reverse Transcription Protocol (One-step SYBR Green I Dye Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

Primer Concentration Optimization Protocol

Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.

Coronavirus qPCR Design Case Study & Related Products to Support SARS-CoV-2 Research

Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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